| 货号: 11586 |
| 产品全名: 人 CD3D 蛋白 |
| 规格: 10/50/100 µg |
| 基因符号 T-cell surface glycoprotein CD3 delta chain;-cell receptor T3 delta chain |
| 目标蛋白: CD3D |
| UNIPROT ID: P04234 |
| 描述: Recombinant human CD3D protein with C-terminal 6xHis tag |
| 背景: The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009] |
| 物种/宿主: HEK293 |
| 分子量: The protein has a predicted molecular mass of 10.4 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His is approximately 15-25 kDa due to glycosylation. |
| 分子特征: CD3D(Phe22-Ala105) 6×His tag |
| 纯化: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| 储存和运输: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD3D Protein, His Tag on SDS-PAGE under reducing condition. |
